Selective Control of Primer Usage in Multiplex One-step Reverse Transcription PCR A Journal Article Review
Polymerase chain reaction or PCR is one of the most promising techniques in Molecular Biology nowadays, and a variant of it is the multiplex RT-PCR or reverse transcription PCR which describes the amplification of the ribonucleic acid (RNA) of interest into its subsequent DNA counterpart. RNA amplification, like many other techniques that involve sub-cellular components, requires a set of complex rules and strict protocols. Hence, effective execution of the multiplex RT-PCR requires two steps, a...